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. 2020 Nov 14;9(11):2478. doi: 10.3390/cells9112478

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) ELISA assay for interaction of recombinant WAK extracellular domains with various cell wall polysaccharides. Results are expressed as conversion of TMB substrate at OD 450 nm which measures the presence of WAK extracellular domain remaining bound to coated polysaccharide using antibody detection of His-tagged recombinant proteins. Each measurement was performed in triplicate. ME = methyl-esterified. MLG = Mixed-linkage glucan. OG = oligogalacturonides. (B) ELISA assay for interaction of recombinant WAK extracellular domains with either A. thaliana or B. distachyon wall fractions enriched for pectin, hemicellulose, or cellulose. Error bars indicate standard error.