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. 2020 Nov 10;21(22):8429. doi: 10.3390/ijms21228429

Figure 7.

Figure 7

AE2 inhibition attenuated the vectorial movement of HaCaT cells. (A) The protein expression of AE2 and β-actin during 2 μM disulfiram (DSF) treatment at 48 h in HaCaT keratinocytes. The β-actin was used as a loading control. (B) Analysis of AE2 intensity in the presence of DSF at 48 h. (C) The cell viability in presence of 2 μM DSF at 24, 48, and 72 h. (D) Time-dependent representative images of migrated HaCaT cells at 4, 24, 48, and 72 h, towards agarose spots containing PBS (pH 7.4) with 500 nM His, with or without 2 μM DSF in the media. The direction of migration across the boundary of the agarose spot is shown as a dashed curve (blue dotted lines). The red dotted lines indicate the lineage of keratinocytes that moved into the spots. (E) Analysis of migration range compared to the control in the presence of 2 μM DSF in the media. Bars indicate the means ± SEM (*** p < 0.001, n = 5).