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. 2020 Nov 10;21(22):8429. doi: 10.3390/ijms21228429

Table 2.

Human reverse transcription-polymerase chain reaction (RT-PCR) primer sequences

Genes Tm (°C) Sequences (5′ → 3′)
Histamine 1 receptor (R) 58 (F) GAC TGT GTA GCC GTC AAC CGG A
(R) TGA AGG CTG CCA TGA TAA AAC C
Histamine 2R 56 (F) TCG TGT CCT TGG CTA TCA C
(R) CTT TGC TGG TCT CGT TCC T
Histamine 3R 70 (F) TCA GCT ACG ACC GCT TCC TGT CGG TCA C
(R) TTG AGT GAG CGC GGC CTC TCA GTG CCC C
Histamine 4R 63 (F) GAA TTG TCT GGC TGG ATT AAT TTG CTA ATT TG
(R) AAG AAT GAT GTG GTG ATG GCA AGG ATG TAC C
GAPDH 62 (F) CAT GGC ACC GTC AAG GCT GAG
(R) CTT GGC CAG GGG TGC TAA GC