Figure 4.

Comparison of radioligand displacement curves at cells co-expressing the H₂R and mGs. (A) [³H]UR-DE257 (50 nM) was displaced by either histamine or famotidine. Presented data are means ± SEM of three independent experiments (n = 3), each performed in triplicate using HEK293T cells stably co-expressing the H₂R-NlucC and NlucN-mGs constructs. (B) Displacement of [³H]UR-DE257 (50 nM) by histamine using HEK293T cells transiently transfected with indicated DNA amounts (in µg) of the H₂R-NlucC and NlucN-mGs constructs 72 h prior to the experiments. Presented data are means ± SEM of three independent experiments (n = 3), each performed in duplicate.