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Molecular characterization of sEVs isolated from normal astrocytes and GBM cell lines. (a) Western blot of cell and sEV lysates. Antibodies directed against proteins enriched in sEVs (HSP70, flotillin-1, CD9) and against a marker for endoplasmic reticulum (calnexin) were used. (b) Mean size of sEVs was determined by nanoparticle-tracking analysis. (c) Transmission electron microscopy images of sEVs. Scale bare is 100 nm. sEVs—small extracellular vesicles.
