Figure 2.

The A2780/S and A2780/CP cells were treated with cisplatin (2 μM) for 24 and 48 h. The intracellular reactive oxygen species (ROS) levels were measured through staining by flow cytometric assay using dihydroethidium (DHE) (A) and 2,7-dichloro-dihydrofluorescein diacetate (DCFH-DA) (B) staining. The A2780/S and A2780/CP cells were treated with cisplatin (2 μM) only or cotreated with NAC. Cell viability was accessed using an MTT assay (B) and cell apoptosis was determined using flow cytometry (C). Results are shown as mean ± SEM (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001).