Figure 5.

(A)—Identification of superoxide dismutase (SOD) isoforms after native polyacrylamide gel electrophoresis (PAGE) separation. SOD was assayed in the presence or absence of inhibitors 1M KCN or 5 mM H2O2; the proteins separated by native PAGE were also immunoblotted using anti-chloroplastic Cu/ZnSOD antibodies. (B)—Zymograms of SOD isoforms. Total soluble proteins isolated from leaves (40 μg proteins/lane) of watered plants (W), drought-stressed plants (D), watered plants treated with SA (WS) or drought-stressed plants treated with SA (DS) were separated by native PAGE and assayed for SOD activity. T0–T4 are different sampling points (see Figure 1). Relative band volumes for individual SOD isoforms are represented by stacked bars.