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. 2020 Nov 12;12(11):3470. doi: 10.3390/nu12113470

Figure 4.

Figure 4

The effects of a fructose-rich diet on the level of GR, lipin-1 PEPCK, and G6Pase in the liver. Groups: control (C), fructose-fed (F). (a) GR protein level in hepatic cytosols and nucleosols, and (b) lipin-1 protein level in hepatic whole cell extracts, were measured by Western blot. Relative integrated optical density of the immunoreactive bands was assessed by ImageQuant software, normalized to equal load controls and expressed as fold of the control. The levels of PEPCK (c) and G6Pase (d) mRNA were determined by qPCR and expressed as fold of the control. The values represent the means ± SEM (n = 9). Statistical significance of differences between experimental groups: * p < 0.05.