Figure 5.

The effects of a fructose-rich diet on the level of GLUT2, fructokinase, and aldolase B in the liver. Groups: control (C), fructose-fed (F). (a) GLUT2 protein level in the hepatic whole cell extracts was measured by immunoblotting. Relative integrated optical density of the immunoreactive bands corresponding to GLUT2 was assessed by ImageQuant software, normalized to β-actin, and expressed as fold of the control. Representative Western blot is shown. The level of fructokinase (b) and aldolase B (c) mRNA relative to β-actin mRNA was determined by SYBR Green real-time PCR and expressed as fold of the control. The values represent the means ± SEM (n = 9). Statistical significance (Student’s t-test) of differences between experimental groups: *** p < 0.001.