Figure 6.

The effects of a fructose-rich diet on the level of NFkB, TNFα, and IL6 in the liver. Groups: control (C), fructose-fed (F). (a) NFkB protein level in hepatic cytosols and nucleosols was measured by Western blot. Relative integrated optical density of the immunoreactive bands corresponding to NFkB was assessed by ImageQuant software, normalized to equal load controls, and expressed as fold of the control. (b) The level of TNFα and IL6 mRNA relative to HPRT mRNA was determined by TaqMan real-time PCR and expressed as fold of the control. The values represent the means ± SEM (n = 9). Statistical significance of differences between experimental groups: ** p < 0.01.