Figure 3.

RR35 regulates bacterial motility of swimming, swarming, and twitching. (A) To test swimming motility, each strain was inoculated on 0.3% XOM2 agar at 28 °C for 7 days, and the diameters of migration zones were analyzed from three experiments. (B) Twitching motility was examined using borosilicate tubes containing 1% agar, followed by 7 days of incubation at 28 °C. (C,D) Swarming motility was examined at the same conditions as for swimming but with 0.5% XOM2 agar, where the RR35 mutant displayed altered swarming motility on plates * indicates that the diameter of RR35 was significantly smaller than that of PXO99A and cRR35 by Duncan’s test (p < 0.001).