Figure 2.

Serum (A) vitamin B₁₂ and (B) folate at fasting (time, 0), hourly until 4 h (time, 1, 2, 3, 4) and the four hour incremental area under the curve (AUC, represented by bar-graphs) following a single multivitamin and mineral supplement ingestion in older and younger adults. Data for multiple time points was compared using general linear mixed model with time as repeated and age as fixed factor. AUC data was compared with general linear univariate analysis of variance with age as fixed factor. There was a main time effect for serum vitamin B₁₂ concentration (p < 0.001) and main time (p < 0.001) and main age effect (p = 0.022) for serum folate. Both vitamin B₁₂ and folate AUC were not different between age groups. ***, p < 0.001, significant increase following supplement intake relative to baseline in both age groups; α, p < 0.05, main age effect.