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. 2020 Nov 17;10(11):1564. doi: 10.3390/biom10111564

Figure 4.

Figure 4

Performance of the anti-EP8 rabbit polyclonal serum for detection of the T. cruzi PS-like protein. (A) SDS-PAGE of CL strain epimastigote whole extract (20 µg) and a corresponding Western blot probed with anti-EP8 serum. Four main bands were identified (arrows). (B) Western blot of whole-cell extracts (20 µg) from epimastigotes under serum deprivation conditions and after treatment with gamma-secretase inhibitors, DAPT (100 µM) and Compound XXI/E (200 µM) after 24 h with polyclonal anti-EP8 serum or anti-β-tubulin antibodies (internal control). Densitometry analysis of the Western blots presented as the ratio of T. cruzi PS-CTF and β-tubulin under nutritional stress (C) and treated with DAPT (100 µM) or compound XXI/E (200 µM) (D). Data represent the mean and standard deviation from at least three independent experiments. * Significant difference using t-test (p < 0.05).