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. 2020 Nov 17;25(22):5375. doi: 10.3390/molecules25225375

Figure 3.

Figure 3

Anti-melanogenic mechanism of lPv-EE and rPv-EE in α-MSH-treated B16F10 cells. (A,B) The promoter binding activity of the transcription factor CREB was analyzed using a reporter gene assay. B16F10 cells were transfected with plasmids driving the expression of CREB-Luc (1 μg/mL) and β-gal (as a transfection control). After 24 h, some cells were treated with 100 nM α-MSH and lPv-EE (400 or 800 μg/mL) or rPv-EE (100 or 200 μg/mL) for 24 h. Luciferase activity was measured using a luminometer. (C–F) Levels of phosphorylated and total CREB, MITF, ERK, p38, JNK, and β-actin proteins were determined in B16F10 cells using phospho-specific or total antibodies for each protein. ** p < 0.01 compared to the normal group and * p < 0.05 compared to the control group.