Figure 4.

Biochemical properties of Fhf1Δ470 are illustrated by C-PAGE analysis. (A) pH optimum. The activity of Fhf1Δ470 was determined at different pH values ranging from 2 to 11. The minus symbol indicates the negative control where no enzyme was added. (B) Temperature optimum. Fhf1Δ470 activity was determined at different temperatures ranging from 20 to 55 °C. (C) Effect of NaCl. The tolerance to NaCl was investigated for concentrations from 15 to 500 mM. (D) Effect of different divalent cations. Wo indicates no ions added to the reaction after EDTA treatment; the minus symbol indicates the negative control where no enzyme was added. All reactions were run for 90 min using 10 g·L⁻¹ of fucoidan extracted from F. evanescens and 0.1 mg·mL⁻¹ of enzyme.