Table 3.
List of oligonucleotide primer sequences and PCR conditions used in this study.
| Primers | Oligonucleotide Sequence (5′–3′) | Genes | Amplicon Size (bp) | Annealing Tm (°C) | References |
|---|---|---|---|---|---|
| 16S rRNA | |||||
| 27F | AGAGTTTGATCATGGCTCAG | 16S rRNA | 1420 | 55 | [49] |
| 1492R | GGTACCTTGTTACGACTT | ||||
| Genus Specific Genes | |||||
| ntrA-F | CATCTCGATCTGCTGGCCAA | ntrA | 90 | 52 | [50] |
| ntrA-R | GCGCGGATCCAGCGATTGGA | ||||
| uidA-F | CTGGTATCAGCGCGAAGTC | uidA | 556 | 52 | |
| uidA-R | AGCGGGTAGATATCACACTC | ||||
| Tuf-F | TCTACTTCACACGTAG | tuf | 240 | 58 | [51] |
| Tuf-R | TTCTAACAGCTCTTCA | ||||
| invA-F | GTGAAATTATCGCCACGTGGCAA | invA | 284 | 64 | [52] |
| invA-R | TCATCGCACCGTCAAAGGAACC | ||||