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. 2020 Nov 17;21(22):8676. doi: 10.3390/ijms21228676

Figure 5.

Figure 5

Dynamics of DOCK8 expression pattern in SK-N-BE(2) and SH-SY5Y over time. (A–D) Representative images of DOCK8 expression at the time points studied (w: weeks) in soft and stiff scaffoldings. Top images correspond to the SK-N-BE(2) cell line cultivated (A) alone and (B) with SW10; the images on the bottom represent the SH-SY5Y cell line cultivated (C) alone and (D) with SW10 cells. Scale bar 50 µm at top left of the first image. Same scale bar is valid for all images. (E–H) Bar chart quantification of DOCK8 staining (% of positive cells) for SK-N-BE(2) cells (E) alone and (F) SK-N-BE(2) cells plus SW10 cells in soft and stiff scaffolds, and SH-SY5Y cells (G) alone and (H) S-SY5Y cells plus SW10 cells in soft and stiff scaffolds. White and black bars: soft and stiff scaffolds, respectively. Dashed lines indicate moving average per stiffness condition. X axis: time in weeks (w) and Y axis: % of positive DOCK8 cells.