Figure 2.

Verification of the interaction between PRCD, TULP1, and TUB by co-immunoprecipitation (co-IP). COS-7 cells were transiently co-transfected with different combinations of PRCD_Myc, TULP1_HA, and TUB_HA. Cell lysates were subjected to co-IP with an anti-Myc tag antibody, and eluted proteins as well as total cell lysates were separated by SDS-PAGE, followed by Western blotting (WB) with anti-HA and anti-Myc tag antibodies. The presence of TULP1 and TUB in the anti-Myc tag immunocomplexes supported their interaction with PRCD in mammalian cells.