Figure 3.

Mapping the interacting regions between PRCD, TULP1, and TUB. (a) Pull-down experiments were performed to test the interaction between PRCD_GST and truncated TULP1_HA and TUB_HA. Samples were analyzed by Western blot with anti-HA and anti-GST antibodies. Within TULP1, the interacting region was mapped to the C-terminal part of the protein (aa 289-543), the region which includes the tubby domain. The N-terminal part of the protein (aa 1-288) did not interact with PRCD. The tubby domain of TUB (aa 248-506) also interacted with PRCD. (b) Shown is a schematic representation of the different pull-down experiments performed to map the interacting regions. The scattered line represents the detected interacting parts.