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. 2020 Nov 17;12(11):3407. doi: 10.3390/cancers12113407

Figure 5.

Figure 5

Induction of IFN-I cascade by DNA sensing in Sting knockout and parental cancer cell lines. LLC1-HER2 cells and Sting knockout counterparts were stimulated in vitro by interferon stimulatory DNA (ISD). Ten hours post treatment, Ifnb (A), Cxcl10 (B), Ccl5 (C) and Isg56 (D) transcripts were assessed by real-time PCR. The relative abundance of target RNAs was evaluated in relation to Actinb transcript. The statistical significances for experiments described in Figure 5 were calculated by Student’s t-test. Panel A, the p-values were 1.2E-5 comparing untreated and treated LLC1-HER2 and 0.01 comparing Sting wild-type vs. knockout cell lines. Panel B, the p-values were 1.2E-5 comparing untreated and treated LLC1-HER2 and 3E-6 comparing untreated and treated LLC1-HER2_SKO. Panel C, 0.003 comparing untreated and treated LLC1-HER2; 0.015 comparing Sting wild-type vs. knockout cell lines. Panel D, 0.0008 comparing untreated and treated LLC1-HER2. Ns indicates statistically not significant differences calculated by Student’s t-test. p <0.05 *; p <0.005 **; p <0.00005 ****.