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. 2020 Oct 30;9(12):637–648. doi: 10.1089/wound.2019.1035

Figure 3.

Figure 3.

Anti-αL treatment alters inflammatory profile in the wound site postburn. (a) Proinflammatory cytokine TNFα and (b) anti-inflammatory cytokine IL-10 expression was assessed by ELISA within the wound tissue of scald burns excised at day 3 and 7 postburn injury. Measurements were normalized to mg wound tissue and expressed as mean pg/mg ± SEM. n = 6. *p < 0.05, #p = 0.074. (c) Macrophage phenotype was determined by fluorescent immunohistochemical analysis of F4/80 (macrophages, green) and YM-1 (M2 macrophages, red) colocalization (merge, orange) within the wound area of 4 μm sections of formalin-fixed and paraffin-embedded scald burns excised at day 3 and 7 postburn injury. Sections were counterstained with DAPI (blue) to visualize the nucleus. Scale bar = 20 μm. Measurements were taken of the number of M2 macrophages (dual F4/80+YM-1+), presented as (d) mean number of dual-positive cells ± SEM within the wound area, presented alongside total macrophage numbers. Solid bar = F4/80+ macrophages, Hatched bar = F4/80+YM-1+ M2 macrophages. n = 6. *p < 0.05. (e) Proscarring TGFβ1 expression was assessed by ELISA within the wound tissue of scald burns excised at day 3 and 7 postburn injury. Measurements were normalized to mg wound tissue and expressed as mean pg/mg ± SEM. n = 6. *p < 0.05. ELISA, enzyme-linked immunosorbent assay; IL-10, interleukin-10; TGFβ1, transforming growth factor beta 1; TNFα, tumor necrosis factor-alpha.