Table 2.
Conjugation Transfer Efficiency of the mcr-9 Gene and Antimicrobial Resistance of Transconjugant TrECJ53-KUFSE-SAL043
| Colistin MIC | Phenotypic resistance | Resistance genes | Concentration (CFU/mL) | Conjugation frequencya | |
|---|---|---|---|---|---|
| KUFSE-SAL043 (Donor) | 4 (μg/mL) | COL, AMP, STR, NAL | mcr-9, aac(6′)-Iaa, blaTEM-1B, qnrS1 | 4.60 × 109 | 2.03 × 10−7 ± 5.42 × 10−8 |
| TrECJ53-KUFSE-SAL043 (Transconjugant) | 2 (μg/mL) | COL, AMP, STR, NAL | mcr-9, aac(6′)-Iaa, blaTEM-1B, qnrS1 | 9.33 × 102 | |
| Escherichia coli J53 AZR (Recipient) | — | — | — | 1.37 × 109 |
a
Conjugation frequency is presented as mean ± standard deviation.
AMP, ampicillin; CFU, colony-forming unit; COL, colistin; MIC, minimum inhibitory concentration; NAL, nalidixic acid. STR, streptomycin.