Figure 4.

Analysis of PDGF2 immunological safety. (A) The BAT was used to evaluate the allergic potential of PDGF2. In vitro activation of basophils in the presence of activating antibodies (first positive control), fMLP (second positive control), a negative control vehicle (water), or PDGF2 was monitored. (B) An analysis was performed via flow cytometry to evaluate the activation levels of selected immune cell subpopulations. Graphs present the expression levels of activation markers on CTLs, Th cells, and NK cells after an incubation with PDGF2 (1 μg/mL). BAT, basophil activation test; CTLs, cytotoxic T lymphocytes; fMLP, N-Formylmethionyl-leucyl-phenylalanine; Th, T helper; NK, natural killer.