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Effect of RHB-104 and its major components on M1/M2 macrophage polarization in MAP-infected macrophages. RT-PCR was used to measure expression of (A) iNOS (M1), and (B) CD206 (M2) in MAP-infected macrophages (24 h) following dose-dependent treatment with RHB-104 (0, 0.5, 1.0, 2.0 μg/mL), CLA (0, 0.32, 0.63, and 1.26 μg/mL), and RIF (0, 0.15, 0.30, 0.60 μg/mL) for 24 h. (C) Illustrates the calculated iNOS/CD206 (M1/M2) ratio for all treatments. * p < 0.05.
