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. 2020 Nov 18;25(22):5392. doi: 10.3390/molecules25225392

Figure 3.

Figure 3

ELISA and virus neutralisation assays. (A) Purified DPP4 protein was used to capture MERS-CoV spike clamp, SARS-CoV-2 spike clamp or a control clamped protein. Clamp-stabilised proteins were detected using a clamp-specific mAb HIV1281. Data shown represent means of duplicate values. (B) DPP4, ACE2, a MERS-specific mAb m336 or a non-specific mAb C05 were used at 20 µg/mL and incubated with MERS-CoV pseudovirus. Percent MERS-CoV inhibition is the percentage reduction in luciferase signal (RLU) compared to virus-only control. N.D. indicates no inhibition detected. Data shown are the mean of duplicate values with error bars representing SD.