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Surface plasmon resonance assays. Purified soluble human ACE2 (A) and DPP4 (B) were exposed to CM5 chips that had been coated with SARS-CoV-2 RBD or spike protein, or were not coated. Experimental data are shown in red. Calculated data fit using a 1:1 binding model are shown in black. Ligands were injected at increasing concentrations of (A) ACE2 at 0.50 nM, 2.5 nM, 12 nM, 62 nM and 310 nM and (B) DPP4 at 1.6 nM, 8.0 nM, 40 nM, 200 nM and 1000 nM.
