Table 2.
DPP4 purification yields and specific activity from five replicate experiments.
| Purification Replicate | Culture Supernatant (L) | Total Protein (mg) | Enzyme Activity (U/mL) | Specific Activity (U/mg) | Final Yield (mg/L Culture) |
|---|---|---|---|---|---|
| 1 | 1 | 1.2 | 45 | 37 | 1.2 |
| 2 | 1.1 | 1.2 | 52 | 43 | 1.1 |
| 3 | 0.98 | 1.5 | 44 | 29 | 1.6 |
| 4 | 0.98 | 1.8 | 61 | 34 | 1.9 |
| 5 | 0.99 | 1.0 | 42 | 40 | 1.0 |
| Mean ± SD | 1.0 ± 0.1 | 1.3 ± 0.3 | 49 ± 7.8 | 36 ± 5.4 | 1.4 ± 0.4 |