Figure 4.

Cytostatic and anti-apoptotic effects of cationic 15 and neutral Pro-functionalized monomeric dendron 13 and bola dendrimers 9a and 11a in T98G populations. (A) Cells were cultured for 96 h after the administration of the agents (1–200 μM) and their numbers were counted with a Coulter counter. (B) T98G cells were treated with 15, 13, 9a and 11a (1–100 μM) and the fraction of apoptotic cells was estimated with ImageStream-AnnexinV/PI assay after 48 h based on the compensated dot-plots, which comprised 50,000 events (Figure S3 in the Supplementary Materials). All data shown as mean ± SD. Statistical significance of the differences tested with Two-way ANOVA, * p < 0.01. Data representative for at least 3 independent experiments (n > 3). Note the lower sensitivity of T98G cells to the analyzed agents in comparison to their U87 counterparts and the lack of straightforward correlation between cytostatic and pro-apoptotic activity of the compounds.