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. 2020 Nov 18;12(11):1106. doi: 10.3390/pharmaceutics12111106

Figure 7.

Figure 7

Effect of His-functionalized bola dendrimers on the bioactivity of T98G glioblastoma cells. (A) T98G cells were cultivated in the presence of 10a or 12a (1–200 μM) for 96 h after administration of the agents and their numbers were counted with a Coulter counter. (B) T98G cells were treated with 10a or 12a (1–100 μM) and the fraction of apoptotic cells was estimated with ImageStream-AnnexinV/PI assay after 48 h (Figure S3 in Supplementary Materials). (C) (Upper panel) T98G cells were treated with 10a and 12a (1–200 μM) and their motility was estimated with time-lapse videomicroscopy after 48 h of treatment (Figure S4 in Supplementary Materials). (Lower panel) Comparison of the morphology and actin (green)/microtubular cytoskeleton (red) architecture of T98G cells cultivated in control conditions and under 10a/12a stress. All data shown as mean ± SD. Statistical significance of the differences tested with two-way ANOVA, * p < 0.01. Data representative for at least 3 independent experiments (n > 3). Scale bar: 50 μm. Note the relatively high cytostatic activity of 12a in the T98G model.