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. 2020 Nov 18;12(11):3413. doi: 10.3390/cancers12113413

Figure 4.

Figure 4

Effect of FL118 on AKT and ERK1/2 expression and phosphorylation/activation in bladder cancer cells. Subconfluent bladder cancer cells were treated with (10, 100 nM) and without (C, control/vehicle) FL118 for 8 h, 16 h, 24 h and 48 h, as shown, followed by Western blot analyses to determine the expression of AKT and ERK1/2 as well as the phospho-AKT (ser473) and phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204). (A) Treatment of HT1376 cells with FL118 does not significantly modulate the expression and phosphorylation of Erk1/2. At the same time, FL118 reduces the expression of pAKT (ser473). (B) Treatment of T24 cells with FL118 enhances the phosphorylation of Akt and Erk1/2. (C) Treatment of UMUC-3 cells with FL118 downregulated the expression of active AKT and ERK1/2. GAPDH presented in (A–C) is internal controls for total protein-loading.