Figure 5.

Sensitivity of bladder cancer cells to FL118 is irrelevant to Top1 level after FL118 treatment. Subconfluent bladder cancer cells in 6-well plates were treated with (10, 50, 100 nM) and without (C, vehicle control) FL118 for 24 h and/or 48 h, as shown, followed by Western blot analyses to determine the expression of Top1. (A) Treatment of HT1376 cells with FL118 resulted in the least inhibition of Top 1 expression after FL118 treatment. (B) Treatment of T24 cells with FL118 resulted in the inhibition of Top 1 expression after FL118 treatment. (C) Treatment of UMUC3 cells with FL118 resulted in the most inhibition of Top 1 expression after FL118 treatment. GAPDH presented in (A–C) is the internal control for total protein-loading.