Figure 8.

Effects of FL118 on Kras pathway-associated ubiquitination (Ub), de-Ub and proteasome-related proteins. HT1376 and UMUC3 cells were treated with FL118 (20 nM) for 24 h and 48 h. Proteomics analyses were then performed as described in the Method section. The data shown here is the effect of FL118 on the Kras pathway-associated Ub, de-Ub and proteasome-related proteins for 24 h (A) and 48 h (B). All data in proteomics analyses are in triple replicates in parallel with triple vehicle controls (refer to Table S1).