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Analysis of the substrate preferences of CPZ determined using the tryptic peptide library. Substrate preferences of CPZ at (A) C-terminal (P1′) and (B) penultimate (P1) positions. The frequency for each amino acid present in P1 or P1′ is indicated for good substrates, weak substrates, and non-substrates. For P1 analysis, only substrates with permissive P1′ amino acids according to the results from (A) were considered.
