Table 1.

Kinetic constants for the hydrolysis of dansyl-Phe-Ala-Arg by different metallocarboxypeptidases (MCPs) with B-like CP activity.

Enzyme	Km (µM)	kcat (s−1)	kcat/Km (µM−1 s−1)
CPZ	1905 ± 360	5.3 ± 0.6	0.0028 ± 0.0008
CPZΔFz	1667 ± 385	6.2 ± 0.8	0.0039 ± 0.0014
CPD domain I (a)	319 ± 37	8.5 ± 0.5	0.027 ± 0.003
CPD domain II (a)	844 ± 139	7.0 ± 0.9	0.008 ± 0.001
CPE (b)	34	13	0.38

^(a) Data obtained from Garcia-Pardo et al. [8]; ^(b) Data obtained from Fricker et al. for CPE purified from bovine pituitary membranes [28].