Table 2.
Good and weak substrates of CPZ identified using the HEK293T peptide library.
| Ratio CPZ/No Enzyme | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Type | Protein Precursor | Peptide Sequence | Z | T | Obs M | Theor M | ppm | 100 nM | 10 nM | 1 nM | 0.1 nM |
| Good | Histidine triad nucleotide-binding protein 1 | Ac-ADEIAKAQVAR | 2 | 1 | 1212.66 | 1212.65 | 14 | 0.02 | 0.94 | 1.08 | 1.00 |
| Good | Eukaryotic translation initiation factor 5A | SAMoxTEEAAVAIKAMAK | 3 | 3 | 1636.81 | 1636.82 | −5 | 0.07 | 0.15 | 0.95 | 0.95 |
| Good | Vimentin | AELEQLKGQGKSR | 4 | 3 | 1442.78 | 1442.78 | −3 | 0.18 | 1.00 | 1.03 | 0.95 |
| Good | Hematological and neurological expressed 1 protein | Ac-TTTTTFKGVDPNSRNSSR | 3 | 1 | 2010.00 | 2009.98 | 13 | 0.31 | 0.89 | 1.24 | 1.07 |
| Weak | Eukaryotic translation initiation factor 5A | NMDVPNIKR | 3 | 2 | 1085.56 | 1085.57 | −6 | 0.45 | n.d. | n.d. | 0.82 |
| Weak | Ubiquitin-60S ribosomal protein L40 | IIEPSLR | 2 | 1 | 826.49 | 826.49 | 0 | 0.60 | 1.00 | 1.13 | 1.04 |
Good substrates, peptides affected with a decrease ≥60% with the highest concentration of enzyme; weak substrates, peptides affected with a decrease ≥20% and <60% with the highest concentration of enzyme; Z, charge; T, number of isotopic tags incorporated into each peptide; Obs M, observed monoisotopic mass; Theor M, theoretical monoisotopic mass; ppm, difference between Obs M and Theor M (in parts per million); Ratio CPZ/no enzyme, the ratio in peak intensity between the sample incubated with enzyme and the sample incubated without enzyme.