Table 3.
Products of CPZ identified using the HEK293T peptide library.
| Protein Precursor | Sequence | Cleaved aa | Z | T | Obs M | Theor M | ppm | Ratio CPZ/No Enzyme | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 100 nM | 10 nM | 1 nM | 0.1 nM | ||||||||
| Heat shock 10 kDa protein 1 | AVGSGSKGKGGEIQPVSV | K | 3 | 3 | 1655.89 | 1655.89 | 2 | 1.30 | 1.16 | 1.28 | 0.93 |
| Peptidylprolyl isomerase A | ADKVPKTAENF | R | 3 | 3 | 1218.62 | 1218.62 | −3 | 1.36 | 1.09 | 1.13 | 1.09 |
| Nucleophosmin | EKTPKTPKGPSSVEDIKA | K | 5 | 5 | 1911.00 | 1911.03 | −18 | 1.46 | 1.00 | 1.23 | 1.15 |
| FK506 Binding Protein | VFDVELL | K | 1 | 1 | 833.45 | 833.45 | 2 | 1.50 | 1.28 | ND | 1.25 |
Products, peptides with an increase >120% with one or more concentrations of enzyme. Cleaved aa, the amino acid cleaved by CPZ to generate the observed peptide. See Table 2 for the rest of abbreviation definitions.