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. 2020 Nov 18;13(11):401. doi: 10.3390/ph13110401

Figure 5.

Figure 5

Colocalization of leptin and LepR with glial fibrillary acidic protein (GFAP) in astrocytes of young (10–12 week) and old (48–52 week) 5XFAD (Tg) mice. Thioflavin-S was used to stain for amyloid plaques. LepR and leptin were detected in astrocytes of both young- (a and e, respectively) and old-Tg mice (l and m, respectively). Astrocytes in old-Tg mice had high expression of GFAP (j,n) compared to young mice (b,f). A high degree of colocalization of GFAP with LepR and leptin in astrocytes was observed in old (k and o, respectively) compared to young-Tg (c and g, respectively) mice. The line expression profile of GFAP and LepR (d) in the astrocytes of young-Tg mice presented similar peaks and troughs along the same relative distance (yellow line). The expression profile of GFAP and leptin (h) shows low levels of leptin (yellow line) in astrocytes of young-Tg mice. Expression profile of GFAP and LepR (l) in the astrocytes of old-Tg mice had similar intensities to young-Tg mice. The intensity profile of GFAP and leptin (p) showed increased expression of leptin in the astrocytes of old-Tg mice. Scale bar = 10μ m.