FIG. 1.

Three-dimensional cerebral organoids and cortical neurons in monolayer differentiated from human iPSCs. (A, B) Protocols used for generation of cerebral organoids and cortical neuron cultures from human iPSCs. (C) Immunohistochemistry of cerebral organoid slices showing antibody staining for neuronal and glial markers as well as for synaptic proteins. Scale bar: 100 μm. (D) Immunocytochemistry of monolayer cortical neuron cultures, with antibody staining of markers for different cortical neuron subtypes. Scale bar: 50 μm. iPSC, induced pluripotent stem cell.