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. 2020 Nov 18;10(1):24–48. doi: 10.1089/wound.2020.1175

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Figure 4. — Different methods to obtain AD-MSCs from adipose tissue. (1) Mechanical and/or enzymatic digestion (collagenase and/or dispase) leads to fragmented adipose tissue. (2) Centrifugation(s) are performed to remove adipocytes and red blood cells (by lysis or Ficoll separation) leading to recovery of the SVF. (3) Isolation of AD-MSCs from SVF is commonly performed by culturing cells into plastic flasks, allowing adherence of AD-MSCs and removal of the nonadherent cell fraction throughout medium changes. (4) The recovery of AD-MSCs requires enzymatic detachment of cells with trypsin, a process that can possibly lead to genetic modification of the cells. AD-MSC isolation can also be performed by cell sorting of the SVF. Color images are available online.