Table 1.
Comparison of different adipose-derived mesenchymal stromal cell isolation methods
| Article | Isolation Method | Cell Concentration (Number/mL) | AD-MSCs (% of total cells) | Comments |
|---|---|---|---|---|
| Zuk et al.32 | Enzymatic (gold standard) | 4,000,000 | 14.3 | AD-MSCs per gram of AT collagenase ± dispase |
| Baptista et al.44 | Mechanical | 240,000 | 5 | PBS washing + centrifugation at 900 g for 15 min |
| Baptista et al.44; | Mechanical | 25,000 | NA | Erythrocyte lysis 15 min + centrifugation at 1200 g for 10 min |
| Battah et al.45 | ||||
| Shah et al.46 | Mechanical | 480,000 | 5.2 | PBS washing under agitation during 1–2 min + centrifugation at 1200 g for 5 min |
| Tonnard et al.47 | Mechanical (nanofat grafting) | [19,000–20,000] | 5.1 | 30 inter-syringe passages + filtration at 500 μM |
| Raposio et al.48 | Mechanical (vortexing) | 125,000 | 5 | PBS washing + vortex 600 vibrations/min + centrifugation at 1600 rpm for 6 min |
| Condé-Green et al.49 | Mechanical | [11,500–23,000] | [6–13] | High-speed centrifugation + vortex for 3 min + conventional centrifugation |
| Markarian et al.50 | Mechanical | 15,000 | NA | Centrifugation at 800 g or 1280 g for 15 min |
| Mechanical | [40,000–50,000] | [1.4–12.8] | Different tryspin concentration + lower centrifugation at 600 g for 10 min | |
| Mashiko et al.51 | Mechanical (micronizing) | [700,000–800,000] | 20 | Passing throw rotating blade device centrifugation at 2300 g for 5 min squeeze vs. emulsifaction47 |
AD-MSC, adipose derived-mesenchymal stromal cell; AT, adipose tissue; PBS, phosphate-buffered saline; NA, not available.