Figure 5.

The effect of MEL and ABT-737 on the calcium retention capacity (CRC) in HL-60 cells. Cells were treated with 0.7 µM ABT-737 (column 2), and 0.2 µM ABT-737 (columns 3) and 1 mM MEL (column 4), and MEL in combination with 0.2 µM ABT-737 (column 5); untreated cells (control, column 1). (a) Ca²⁺ fluxes in HL-60 cells permeabilized with digitonin (0.007%). Arrows show the times at which CaCl₂ (50 nmol of Ca²⁺ per mg of protein) was added; (b) Quantitative analysis of Ca²⁺ retention capacity corresponding to the threshold concentration of Ca²⁺. “+” means the presence of MEL The data are presented as the means ± S.D. of five separate experiments. * p < 0.05 significant difference relative to the control (untreated cells), ^# p < 0.05 significant difference compared to MEL alone (column 4).