Figure 2.

Cryo-TEM images of PLGA-DY-635 NPs (A) and PLGA-DY-635 (BIM-I) NPs (B) purified via crossflow filtration (white arrow heads point to NPs); particle analysis from cryo-TEM images was carried out by ImageJ software and histograms represent the number distribution (n = 80) (C); Diameter of the NPs purified via the crossflow filtration estimated by the different analytical methods (D). The size variation bars represent the standard deviation of sizes calculated from the respective distributions of sizes, i.e., of the number-based hydrodynamic diameter distributions from a representative distribution of the five DLS measurements, the hydrodynamic diameter distributions derived from differential distributions of sedimentation coefficients, and the mean apparent geometrical NP diameter from cryo-TEM measured by ImageJ.