Table 1.
Size, PDI, and zeta potential of the NPs as measured by DLS.
| NP Formulation | Purification Method | dH (nm) a ± SD | dH (nm) b ± SD | dH (nm) c ± SD | PDI ± SD | ζ (mV) ± SD |
|---|---|---|---|---|---|---|
| PLGA-DY-635_U | Ultrafiltration | 120 ± 24 | 133 ± 72 | 66 ± 19 | 0.15 ± 0.07 | −20 ± 8 |
| PLGA-DY-635_C | Crossflow filtration | 65 ± 17 | 50 ± 26 | 49 ± 3 | 0.15 ± 0.03 | −20 ± 0 |
| PLGA-DY-635 (BIM-I)_U | Ultrafiltration | 92 ± 3 | 73 ± 2 | 28 ± 8 | 0.22 ± 0.05 | −7 ± 10 |
| PLGA-DY-635 (BIM-I)_C | Crossflow filtration | 63 ± 7 | 37 ± 3 | 22 ± 2 | 0.30 ± 0.08 | −9 ± 2 |
a–Z-average intensity distribution; b–volume distribution; c–number distribution. U denotes NPs purified via ultrafiltration, C denotes NPs purified via crossflow filtration. The standard deviation (SD) of samples purified via ultrafiltration represents the error between different formulated batches (n = 8). The standard deviation of dH, PDI, and ζ of the samples purified via crossflow represents the error between five DLS measurements.