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Submerged normal human bronchial epithelial (NHBE) cells were stimulated with poly(I:C) (25 µg/mL), IFN-γ (100 ng/mL), and IL-4 (1 ng/mL) for 24 h. (A) Cell lysates were harvested for RNA extraction to analyze 15-LOX-1 mRNA expression by RT-PCR (n = 3–6). Data shown are the means ± SEM of four independent experiments. ** p < 0.01 when compared to non-stimulated cells.
