Table 2.
Schematic of the xLFIA and x2LFIA formats used in this study.
| Reporter | Capture | |||||||
|---|---|---|---|---|---|---|---|---|
| Format | Adsorbed on GNP | Adsorbed on GNS | Optical Density (Ratio) b | Test Line 1 (T1) | Test Line 2 (T2) | Control Line (C) | ||
| xLFIA | A | gp36 | biotin a | gp41 | 2.5 (1 + 0.5 + 1) | gp36 | gp41 | avidin |
| B | gp36 | biotin a | gp41 | 2.5 (1 + 0.5 + 1) | gp36/gp41 | - | avidin | |
| x2LFIA | 1 | gp36 | biotin a | gp41 | 2.5 (1 + 0.5 + 1) | anti-IgM | protein G | avidin |
| 2 | protein G | anti-IgM | 3 (1.5 + 1.5) | gp36 | gp41 | protein G | ||
| 3 | gp36 | gp41 | anti-IgM | 3 (1 + 1 + 1) | gp36 | gp41 | protein G | |
a to form the control line, b the probes were mixed to reach the optical density in variable ratio.