Figure 2.

Level of citrullinated histone H3 in PMA-stimulated neutrophils from control and myeloma-bearing mice. Neutrophils were isolated from the BM of (a) DP42 myeloma-bearing or control tumor-free mice (n = 5 and n = 4, respectively) or (b) 5TGM1-bearing or control tumor-free mice (n = 3 for each group) and stimulated with 200 nM PMA for 4 h. Cells were then collected and subjected to Western blotting with antibodies against citrullinated histone H3. Equal loading was confirmed by re-probing membranes with antibodies against β-actin. Western blots are shown in Figure S1. Left, representative Western blots are shown. Right, densitometry results presented as mean ± SEM values are shown.