Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 18;12(11):3424. doi: 10.3390/cancers12113424

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Quantitative citrullinated histone H3 bound to DNA (CitH3DNA) binding assay for detection of NETs. High binding 96-well plates were coated with anti-histone H3cit antibodies. (a) Neutrophils were isolated from BM of myeloma-bearing mice and stimulated in vitro with indicated concentrations of PMA for 4 h. NET fractions were collected from each condition and 100 μL were loaded per well. (b) Indicated amount of genomic DNA was added per well. (a,b) Fluorescence of SYTOX Green nucleic acid stain was measured using a Victor X3 plate reader. RFU—relative fluorescence units.