Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Quantification of citrullinated histone H3 for detection of IL-1β-induced NETosis. Neutrophils were isolated from the BM of tumor-free mice (n = 3) and stimulated in vitro with 200 ng/mL of murine IL-1β for 6 h. (a) Formation of NETs was evaluated by microscopy and quantified. (b) NET fractions were collected and used in the CitH3DNA binding assay. Mean ± SEM values obtained from neutrophils isolated from 3 individual mice are shown.
