Table 1.
Cryo-EM data collection and processing.
| Data Collection | AAV5 (Uncomplexed) | AAVR-AAV5 Complex |
|---|---|---|
| Magnification | 81,000× | 64,000× |
| Voltage | 300 kV | 300 kV |
| Electron exposure | 31.7 e−/Å2 | 32.9e−/Å2 |
| Defocus range | −0.7 to −2 μm | −0.7 to −2.5 μm |
| Pixel size | 0.530 Å | 0.664 Å |
| (on refinement vs. atomic model:) | 0.533 Å | 0.665 Å |
| Data processing | ||
| Motion correction | Relion 3.1-beta | Relion 3.0 |
| CTF estimation | CTFFIND-4.1 | CTFFIND-4.1 |
| Symmetry imposed | I1 | I1 |
| Initial particle images | 672,106 | 168,275 |
| Final particle images | 373,426 | 159,673 |
| Map resolution | 2.13 Å | 2.5 Å |
| FSC threshold | 0.143 | 0.143 |