Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 18;21(22):8726. doi: 10.3390/ijms21228726

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Tools for investigating the intra-axonal roles of miRNAs. (a) Schematics for distal axons isolation: Campenot chambers, microfluidic devices or manual removal of the explants in case of organoculture. (b) Techniques for studying miRNA function: by loss of function (LOF) approach blocking miRNA function (1–3), by gain of function (GOF) approach increasing miRNAs level with mimics (4); by blocking miRNA target recognition, masking the miRNA-responsive element (MREs) with a target protector (5); by studying with reporters the translational events potentially regulated by the miRNA under study (6,7); by measuring the miRNA activity using sensor plasmids (8). Abbreviations: ORF, open reading frame; ASOs, antisense oligonucleotides.